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KMID : 0545120090190091013
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 9 p.1013 ~ p.1018
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Characterization of Bacillus polyfermenticus KJS-2 as a Probiotic
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Kim Kang-Min
Kim Myo-Jeong
Kim Dong-Hee
Park You-Soo
Kang Jae-Seon
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Abstract
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The identification and characterization of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) was conducted using TEM, an API 50CHB kit, 16S rDNA sequencing, a phylogenetic tree, and catalase and oxidase testing. The conversion rate of glucose to lactic acid by B. polyfermenticus KJS-2 was found to be 60.71±4.9%. In addition, treatment of B. polyfermenticus KJS-2 with artificial gastric juice (pH 2.0) and bile acid (pH 6.5) for 4 h resulted in a final viability of 140±7.9% and 108±3.5%, respectively. Finally, the results of adhesion experiments using Caco-2 cells revealed that the adherence of B. polyfermenticus KJS-2 to Caco-2 cells was approximately 65±0.6%.
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KEYWORD
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Bacillus polyfermenticus KJS-2, probiotic, characterization, industrial application
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